A lesson on the polymerase chain reaction with a starter, aims and animation links. It explains what goes into a PCR machine, the heating and cooling steps, how primers are designed, why a thermostable DNA polymerase is needed and why contamination must be avoided, finishing with the uses of PCR.
What this presentation covers
- what PCR is for
- the reaction mix: DNA, nucleotides, primers and polymerase
- separating strands at 95 degrees
- designing primers
- thermostable DNA polymerase
- avoiding contamination
- uses of PCR
Level: A Level
Slides: 14
Format: PowerPoint (.pptx)